Journal: ACS Physical Chemistry Au

Article Title: Quantifying Microsecond Solution-Phase Conformational Dynamics of a DNA Hairpin at the Single-Molecule Level

doi: 10.1021/acsphyschemau.3c00066

Figure Lengend Snippet: (a) Representative photon-arrival correlation plots for three single-molecule events. The displayed plots were created with a Δ T window of 500–600 μs, and other plots ( Section SI.14 ) were created for shorter and longer Δ T windows. (b, c) Resulting lifetime plots from an inverse Laplace transform of the correlation plots in panel (a). The peak’s x and y -locations correspond to the lifetime of the first and second photon, respectively, while the peak height corresponds to the relative number of photon pairs observed with these lifetimes (the lifetime distribution). (b) Result of a 2D correlation plot with a shorter Δ T (shown in Section SI.14 ), while panel (c) is the result of an inverse Laplace transforming of panel (a). (d) Lifetime distributions from the analysis of the 2D lifetime plots taken at numerous Δ T windows. These lifetime distributions are empirically derived for each individual molecule and give the filter functions for later fFCS analysis.

Article Snippet: A characteristic macrotime interval (corresponding to molecular dynamics in a temporal range of interest, Figure b, Δ T ) is selected, and photon pairs whose macrotimes fall within a time window Δ T ± ΔΔ T /2 have their microtimes correlated with each other to create a 2D correlation plot ( Figure b, e).

Techniques: Derivative Assay